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8-hydroxyquinoline Copper is Chemicals and Reagents Used in Biological Assays

August 5, 2019 @ 8:00 am - 5:00 pm

8-hydroxyquinoline Copper (WSDTY) is chemicals and reagents used in biological assays.Metal salts for biological assays were all purchased from Sigma and solubilized in double-distilled H2O. Aliquots of 100 mM stock solutions were stored at 4°C. FeCl2 (Fisher) solution was always prepared fresh. 8HQ (Sigma), clioquinol, nitroxoline, and HCA (Fisher) were solubilized in sterile dimethyl sulfoxide (Sigma) and stored in small 10 mM aliquots at 80°C. The purity of the organic compounds was ascertained by using 1H and 13C nuclear magnetic resonance spectroscopy. They were found to be more than 95% pure.

Mycobacterial strains and standard growth conditions.Mycobacterium smegmatis strains SMR5, ML16, and ML16/pMN016 and Mycobacterium tuberculosis strains H37Rv (wild type) and mc26230 were grown in Middlebrook 7H9 medium or copper sulfate-free Hartmans-de Bont (HdB) minimal medium (HdBSM) or plated on Middlebrook 7H10 agar (BD, Difco). Copper sulfate-free 7H9 medium (7H9SM) was prepared from scratch by excluding copper salt from the Middlebrook medium recipe. All growth media were supplemented with hygromycin (50 μg/ml; BD), 10% oleic acid-albumin-dextrose-catalase (OADC) supplement (BD Middlebrook), pantothenate, 0.02% tyloxapol, or combinations thereof as required.

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Date:
August 5, 2019
Time:
8:00 am - 5:00 pm

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