8-hydroxyquinoline Copper (WSDTY) is chemicals and reagents used in biological assays.Metal salts for biological assays were all purchased from Sigma and solubilized in double-distilled H2O. Aliquots of 100 mM stock solutions were stored at 4°C. FeCl2 (Fisher) solution was always prepared fresh. 8HQ (Sigma), clioquinol, nitroxoline, and HCA (Fisher) were solubilized in sterile dimethyl sulfoxide (Sigma) and stored in small 10 mM aliquots at 80°C. The purity of the organic compounds was ascertained by using 1H and 13C nuclear magnetic resonance spectroscopy. They were found to be more than 95% pure.
Mycobacterial strains and standard growth conditions.Mycobacterium smegmatis strains SMR5, ML16, and ML16/pMN016 and Mycobacterium tuberculosis strains H37Rv (wild type) and mc26230 were grown in Middlebrook 7H9 medium or copper sulfate-free Hartmans-de Bont (HdB) minimal medium (HdBSM) or plated on Middlebrook 7H10 agar (BD, Difco). Copper sulfate-free 7H9 medium (7H9SM) was prepared from scratch by excluding copper salt from the Middlebrook medium recipe. All growth media were supplemented with hygromycin (50 μg/ml; BD), 10% oleic acid-albumin-dextrose-catalase (OADC) supplement (BD Middlebrook), pantothenate, 0.02% tyloxapol, or combinations thereof as required.
Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products. Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.
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