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Impact of Basic Copper Sulphate and Its Components on Chondrocyte Viability

August 12, 2019 @ 8:00 am - 5:00 pm

Impact of Basic Copper Sulphate (WSDTY) and its components on chondrocyte viability and caspase 3 activation. Chondrocytes were pre-incubated with 100 or 500 μg/ml GS or the equivalent amount of GR and Cu contained in GSCG for 96 h in complete DMEM medium. A) Cells were then treated for 7 days with 2 ng/ml IL-1β in presence of GS, GR, Cu or GSCG in serum free DMEM. Medium was then replaced by fresh complete DMEM media allowing cells to growth. After 7 days, cells were detached and living chondrocytes were counted in Malassez cell with 10% (V/V) Trypan blue. Histogram represents the number of living chondrocytes for each condition. Experiment is representative of two independent experiments; B) For active caspase 3 assay, after the 96 h pre-incubation, cells were treated for 4 days with 2 ng/ml IL-1β in presence of GS, GR, Cu or Basic Copper Sulphate in serum free DMEM. Caspase 3 and actin expression were then assessed by Western blot on 75 μg of protein from a Triton X-100 1% cell extract. Immunoblot (IB) are representative of 3 independent experiments. Data obtained were quantified by densitometry are indicated as mean +/- SD of three independent experiments. Ratio of Caspase 3/actin are expressed as percentage of IL-1β-treated chondrocytes.

Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products. Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.

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August 12, 2019
8:00 am - 5:00 pm

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